PIERCE 15121-Reacti-Bind Streptavidin Coated Polystyrene Strip
產(chǎn)品名稱：Reacti-Bind Streptavidin Coated Polystyrene Strip Plates, wi
產(chǎn)品貨號：PIERCE 15121
產(chǎn)品規(guī)格：5 PLATES
產(chǎn)品價格：2086
產(chǎn)品說明書地址：http://www.piercenet.com/
銷售直線：021-31322337、（何）
Introduction
The Thermo Scientific Pierce Streptavidin Coated Plates are made of polystyrene and are ideal for binding assays using biotinylated molecules. These plates are especially advantageous when direct adsorption to polystyrene plates denatures antibodies or the target molecule. Streptavidin has no carbohydrate groups and an isoelectric point of 5-6, resulting in low nonspecific interactions. The streptavidin coated plates are available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.
Example ELISA Procedure
The following protocol describes a generalized enzyme-linked immunosorbent assay using a biotinylated capture antibody. Please see the reference list for other possible applications using streptavidin-coated microplates.
A. Materials Required
• Wash Buffer: Tris-buffered saline （25mM Tris, 150mM NaCl; pH 7.2; Product No. 28376）, 0.1% BSA, 0.05% Tween®-20 Detergent; alternatively, use Thermo Scientific Blocker BSA （Product No. 37520） supplemented with 0.05% Tween-20
• Biotinylated capture antibody adjusted to 10μg/mL, or other appropriate concentration, with Wash Buffer
• Antigen adjusted to appropriate concentration with Wash Buffer
0463.3
