PIERCE 15128Reacti-Bind （tm） NeutrAvidin （tm） Coated Polystyrene Strip
產品名稱：Reacti-Bind （tm） NeutrAvidin （tm） Coated Polystyrene Strip
產品貨號：PIERCE 15128
產品規格：5 PLATES/EA
產品價格：1910
產品說明書地址：http://www.piercenet.com/
Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are pre-blocked and ready-to-use polystyrene 96-well and 384-well plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.
Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are available in 96-well and 384-well plate formats and pre-blocked with BSA or SuperBlock Blocking Buffers. These Pierce Biotin-Binding Plates are available in clear, white and black plates can be used with colorimetric, chemiluminescent and fluorescent detection methods, respectively.
Highlights:
- Easy and gentle immobilization of biotin-containing conjugates
- Low nonspecific binding
- No denaturing of the protein component of a conjugate upon binding
- Ideal for binding small biotinylated hydrophilic molecules （e.g., peptides） that typically exhibit poor binding to polystyrene
- Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer
Additional Highlights of NeutrAvidin Coated Plates:
- Less expensive than streptavidin coated plates
- Lowest nonspecific binding properties of all biotin-binding proteins
Product Details:
| Plate Characteristics for Standard Capacity Biotin-Binding Plates. | ||||
| Biotin-Binding Protein | Plate Type | Coat Volume** | Block Volume** | Approximate Binding Capacity |
| NeutrAvidin | 96-Well, 8-Well Strip | 100µL/well | 200µL/well | ~15pmol biotin/well |
| 384-Well | 50µL/well | 100µL/well | ~10pmol biotin/well | |
| Streptavidin | 96-Well, 8-Well Strip | 100µL/well | 200µL/well | ~5pmol biotin/well |
| 384-Well | 50µL/well | 100µL/well | ~4pmol biotin/well | |
| ** The coating and blocking volumes give here are typical. Additional variations are also available. | ||||
Streptavidin and NeutrAvidin Protein are not glycosylated which prevents nonspecific lectin-binding observed with avidin coated plates. Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin has an isoelectric point of 5-6, resulting in low nonspecific interactions. NeutrAvidin Protein has a near-neutral pI （6.3）, resulting in even lower nonspecific interactions. NeutrAvidin Protein has an additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells. NeutrAvidin Protein has the lowest nonspecific binding among the known biotin-binding proteins.
Related Products and Links:
High Binding Capacity Biotin-Binding Plates
High Sensitivity Biotin-Binding Plates
SuperBlock Blocking Buffer – or browse all blocking buffers
Secondary Antibody Selection Guide
ELISA Substrate Selection Guide
ELISA Accessories – microplate sealing tape, reagent reservoirs, microplates
銷售直線：021-31322337、（何）
