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產(chǎn)品名稱：TCEP HCl, Tris（2-Carboxyethyl） Phosphine Hydrochloride
產(chǎn)品貨號：PIERCE 20490
產(chǎn)品規(guī)格：1 GM
產(chǎn)品價(jià)格：566
產(chǎn)品說明書地址：http://www.piercenet.com/
| 20490 | TCEP HCl, Tris（2-Carboxyethyl） Phosphine Hydrochloride | 1 GM | |
| 20491 | TCEP HCl, 10 g. Trisphosphine | 10 G |
Thermo Scientific Pierce TCEP-HCl is pure, crystalline Tris（2-carboxyethyl）phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.
TCEP selectively and compley reduces even the most stable water-soluble alkyl disulfides over a wide pH range. Reductions frequently require less than 5 minutes at room temperature. TCEP is non-volatile, odorless, and unlike most other reducing agents, is resistant to air oxidation. Compared to DTT, TCEP is more stable, more effective, and able to reduce disulfide bonds at lower pHs.
Highlights:
- Odorless – reduce proteins at your desktop; contributes to a healthier lab environment
- Specific – selective and complete reduction of even the most stable water-soluble disulfides
- Simple – effective reduction at room temperature and pH 5 in less than five minutes
- Stable – resistant to air oxidation; nonvolatile and nonreactive toward other functional groups found in proteins
Advantages of TCEP-HCl over traditional alternatives for reducing disulfides:
- Odorless – Unlike DTT or BME, TCEP is odor-free, so reductions can be carried out conveniently on the bench top.
- Stable in air – The inherent stability of the TCEP moiety eliminates the need for any special precautions to avoid oxidation when handling, using or storing TCEP.
- Efficient – For most applications, 5-50 mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.
- Compatible – With TCEP, removal of the reducing agent is not necessary prior to most applications, （e.g. histidine-tagged protein purification, maleimide conjugations）.
Product Details:
The ability of trialkylphosphine compounds to reduce protein disulfide bonds have been known for many years.1,2 Phosphines are stable in aqueous solution, selectively reduce disulfide bonds, and are essentially nonreactive toward other functional groups commonly present in proteins.2 Trialkylphosphines, however, were hindered by their instability in water and their disagreeable odor. These obstacles were overcome by discovery of tris（2-carboxyethyl）phosphine （TCEP）.3-17
