PIERCE，Sulfo-SMCC, 1 g.
- 型號： 22122
- 更新時間： 2024-05-31
- 上一個：21857PIERCE，N-Succinimidyl 3-（2-Pyridyldithio）Propionat
- 下一個：22305PIERCE，MPBH: 4-（4-N-Maleimidophenyl）Butyric Acid H
訂貨：，，
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Sulfo-SMCC介紹
Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
Sulfo-SMCC現貨供應
PIERCE | 22122 | Sulfo-SMCC, 1 g. | 1 G |
PIERCE | 22305 | MPBH: 4-（4-N-Maleimidophenyl）Butyric Acid Hydrazide Hydrochl | 50 MG. |
PIERCE | 22307 | Sulfo-EMCS- N-（E-Maleimidocaproyloxy） Sulfosuccinimide Este | 50 MG |
Sulfo-SMCC品牌介紹
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Sulfo-SMCC僅用于科研
Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.
Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Sulfo-SMCC Highlights
- Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
- Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
- Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
- High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
- Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C。
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