PIERCE，DSP （Lomant's Reagent）
- 型號： 22586
- 更新時間： 2024-05-31
- 上一個：22585PIERCE，DITHIOBIS （SUCCINIMIDYL PROPIONATE），（DSP）
- 下一個：22589PIERCE，（SULFO-SANPAH） SULFOSUCCINIMIDYL 6-（4'-AZID
訂貨：，，
DSP （Lomant's Reagent）介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
DSP （Lomant's Reagent）現(xiàn)貨供應(yīng)
PIERCE | 22586 | DSP （Lomant's Reagent） , 50 mg | 50 MG |
PIERCE | 22589 | （SULFO-SANPAH） SULFOSUCCINIMIDYL 6-（4'-AZIDO-2'-） NITROPHENY | 50 MG |
PIERCE | 22622 | Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg microtubes | 8 X 2 MG |
DSP （Lomant's Reagent）品牌介紹
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DSP （Lomant's Reagent）僅用于科研
Thermo Scientific Pierce DSP （Lomant's Reagent） is an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm.
DSP contains an amine-reactive N-hydroxysuccinimide （NHS） ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. DSS, the non-cleavable analog of DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
DSP Highlights
- Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- DSP crosslinker is membrane permeable, so intracellular crosslinking can be done
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