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The Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff （PAS） method.

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins （glycoproteins） that have been separated by polyacrylamide gel electrophoresis （PAGE）. A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff （PAS） reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

• Specific for glycoproteins – periodic acid-Schiff （PAS） method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
• Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours （half the time required for other staining methods）
• Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
• Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16µg horseradish peroxidase per band
• Controls included – complete kit includes positive and negative control proteins （HRP and STI, respectively）.
• Gels or membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes