重組人成纖維細胞生長因子-22（rHuFGF-22）
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- 更新時間： 2024-05-23
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產(chǎn)品介紹
| CYT-428 | 重組人成纖維細胞生長因子-22（rHuFGF-22） | 2μg/10μg/1mg | Prospec |
| Background : | |
 | Description : |
| Recombinant Human FGF-22 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17173 Dalton. Recombinant FGF-22 is purified by chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered white lyophilized powder. | |
| Formulation: |
| The sterile protein powder is lyophilized with no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Recombinant Human FGF-22 sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized rHuFGF-22 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuFGF-22 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Ser-Ala-Ser. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| Measured in a cell proliferation assay using IEC6 cell line （20ng/ml can stimulate the cell proliferation of IEC6 cell line）. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Recombinant Human FGF-22. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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